1J4U

Structure of Artocarpin Complexed with Me-alpha-Mannose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 61
Unit cell lengths	129.200, 129.200, 78.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.900
R-factor	0.222 *
Rwork	0.222
R-free	0.25800
RMSD bond length	0.010
RMSD bond angle	3.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.157	0.416
Total number of observations	31710 *
Number of reflections	15219
Completeness [%]	91.3	83.7
Redundancy	2.1	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4	293	PBS Buffer, methoxy PEG 350, 10Mg/ml protein, pH 7.4, vapor diffusion, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)	pH7.4
2	1	drop		0.15 (M)
3	1	drop	methoxy PEG350	40 (%)
4	1	reservoir	methoxy PEG350	40 (%)