1J4T

Structure of Artocarpin: a Lectin with Mannose Specificity (Form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	87.690, 72.190, 92.630
Unit cell angles	90.00, 101.15, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.191 *
Rwork	0.191
R-free	0.25800
RMSD bond length	0.010
RMSD bond angle	1.500 *
Data reduction software	XDS
Data scaling software	XDS
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.089	0.379
Total number of observations	133210 *
Number of reflections	42342
Completeness [%]	96.1	56
Redundancy	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4	293	PBS Buffer, 20% PEG 1450, 10Mg/ml protein, pH 7.4, vapor diffusion, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)	pH7.4
2	1	drop		0.15 (M)
3	1	drop	methoxy PEG350	40 (%)
4	1	reservoir	methoxy PEG350	40 (%)