1J4J
Crystal Structure of Tabtoxin Resistance Protein (form II) complexed with an Acyl Coenzyme A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-24 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.667, 66.593, 53.599 |
| Unit cell angles | 90.00, 103.68, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.550 |
| Rwork | 0.188 |
| R-free | 0.25400 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.810 |
| Data reduction software | DENZO (2000) |
| Data scaling software | SCALEPACK (2000) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.350 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.056 | 0.132 |
| Number of reflections | 15493 | |
| <I/σ(I)> | 18.7 | |
| Completeness [%] | 99.4 | 98.7 |
| Redundancy | 3 | 4.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP/VAPOR DIFFUSION | 8 | 291 | PEG 4000, sodium acetate, Tris-HCL, pH 8.0, HANGING DROP/VAPOR DIFFUSION, temperature 291.0K |
