1J3A
Crystal structure of ribosomal protein L13 from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Detector technology | CCD |
| Collection date | 2002-04-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.082, 51.542, 64.198 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.2094 |
| Rwork | 0.209 |
| R-free | 0.22750 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.170 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.700 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 17561 | |
| Completeness [%] | 94.8 | 77.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.1M Phosphate-citrate, 20% PEG8000, 0.2M NaCl, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
