1J2Z

Crystal structure of UDP-N-acetylglucosamine acyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9794
Spacegroup name	P 63 2 2
Unit cell lengths	90.686, 90.686, 148.203
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 2.100
R-factor	0.222
Rwork	0.222
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lxa
RMSD bond length	0.005
RMSD bond angle	24.200 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.210
High resolution limit [Å]	2.100	2.100
Number of reflections	21519 *
<I/σ(I)>	6.9	1.8
Completeness [%]	99.1 *	99.8
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	Lee, B.I., (2002) Acta Cryst., D58, 864. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)