1J2P
alpha-ring from the proteasome from archaeoglobus fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-10-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 193.755, 214.226, 43.963 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.450 * |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | proteasome from thermoplasma acidophilum |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.520 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.630 |
| High resolution limit [Å] | 2.450 * | 2.580 |
| Rmerge | 0.043 * | 0.367 * |
| Total number of observations | 1248529 * | |
| Number of reflections | 67959 | |
| Completeness [%] | 97.8 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 * | 24 * | 3M 1,6-Hexandiol, 85mM Magnesiumchlorid, 100mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | 100 (mM) | pH4.6 | |
| 3 | 1 | reservoir | PEG400 | 9 (%(w/v)) | |
| 4 | 1 | reservoir | 80 (mM) |
