1J2P

alpha-ring from the proteasome from archaeoglobus fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-04
Detector	MARRESEARCH
Wavelength(s)	1.1
Spacegroup name	P 21 21 2
Unit cell lengths	193.755, 214.226, 43.963
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.450 *
R-factor	0.257
Rwork	0.257
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	proteasome from thermoplasma acidophilum
RMSD bond length	0.010 *
RMSD bond angle	1.520 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.630
High resolution limit [Å]	2.450 *	2.580
Rmerge	0.043 *	0.367 *
Total number of observations	1248529 *
Number of reflections	67959
Completeness [%]	97.8	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6 *	24 *	3M 1,6-Hexandiol, 85mM Magnesiumchlorid, 100mM Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	reservoir		100 (mM)	pH4.6
3	1	reservoir	PEG400	9 (%(w/v))
4	1	reservoir		80 (mM)