1J22

Crystal structure of archaeal XPF/Mus81 homolog, Hef from Pyrococcus furiosus, nuclease domain, selenomet derivative

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-01
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00, 0.97, 0.9791, 0.9794
Spacegroup name	P 31 2 1
Unit cell lengths	69.795, 69.795, 58.509
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.800
Rwork	0.223
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.005 *
RMSD bond angle	1.100 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.052	0.118
Number of reflections	15484
<I/σ(I)>	17.3
Completeness [%]	97.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	8	20 *	Tricine-NaOH, NaCl, NaN3, subtilisin, pH 8.0, MICRODIALYSIS, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	60 (mg/ml)
2	1	2	tricine-NaOH	100 (mM)	pH8.0
3	1	2		300 (mM)
4	1	2		0.025 (%)