1J1X

Crystal Structure of HyHEL-10 Fv mutant LS93A complexed with hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-25
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	56.616, 56.616, 234.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
Rwork	0.189
R-free	0.21700 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.280
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.900	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.067	0.156
Number of reflections	36671
<I/σ(I)>	7.4	4.5
Completeness [%]	99.9	99.9
Redundancy	14.1	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6	20 *	Kondo, H., (1999) J.Biol.Chem., 274, 27623. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)	pH7.6-7.8
2	1	reservoir	PEG6000	9-11 (%(w/v))
3	1	reservoir	glycerol	15 (%)
4	1	reservoir	MPD	7-9 (%(w/v))