1J18
Crystal Structure of a Beta-Amylase from Bacillus cereus var. mycoides Cocrystallized with Maltose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.808, 89.331, 65.394 |
| Unit cell angles | 90.00, 102.32, 90.00 |
Refinement procedure
| Resolution | 29.000 - 2.000 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.22100 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.183 * |
| Number of reflections | 43133 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG 6000, ammonuim sulfate, maltose, acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | acetate | 100 (mM) | pH4.6 |
| 3 | 1 | reservoir | PEG6000 | 16-18 (%(w/v)) | |
| 4 | 1 | reservoir | ammonium sulfate | 7 (%(w/v)) | |
| 5 | 1 | reservoir | maltose | 50 (mM) | |
| 6 | 1 | reservoir | acetate | 100 (mM) | pH4.6 |
