1J0Y
Beta-amylase from Bacillus cereus var. mycoides in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.900, 112.900, 146.200 |
| Unit cell angles | 90.00, 105.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| Rwork | 0.179 |
| R-free | 0.23600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5bca |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.476 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.500 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.214 |
| Total number of observations | 243229 * | |
| Number of reflections | 135775 | 12350 * |
| Completeness [%] | 72.8 | 53.1 |
| Redundancy | 1.8 * | 1.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | Oyama, T., (1998) Protein Pept.Lett., 5, 349. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | ammonium chloride | 50 (mM) | |
| 3 | 1 | reservoir | PEG6000 | 6-8 (%(w/v)) | |
| 4 | 1 | reservoir | ammonium chloride | 50 (mM) |
