1J02

Crystal Structure of Rat Heme Oxygenase-1-Heme Bound to NO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-10-08
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	65.370, 65.370, 121.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.202
Rwork	0.201
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ivj
RMSD bond length	0.005
RMSD bond angle	18.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.052	0.346
Number of reflections	32251
<I/σ(I)>	18.1
Completeness [%]	99.8 *	99.5 *
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Sugishima, M., (2002) J.Biol.Chem., 277, 45086. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium formate	4 (M)
2	1	drop	protein	18 (mg/ml)
3	1	drop	potassium phosphate	50 (mM)	pH7.0
4	1	drop	sodium azide	5 (mM)