1IZR
F46A mutant of bovine pancreatic ribonuclease A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.999995 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.241, 64.241, 63.057 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.500 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fs3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 * | 0.128 |
| Number of reflections | 24207 | |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 98.8 | 95.8 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 25 * | ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | pH6.0 |
| 4 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 5 | 1 | reservoir | 2 (M) |
