1IZP

F46L mutant of bovine pancreatic ribonuclease A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-12
Detector	ADSC QUANTUM 4
Wavelength(s)	0.700000
Spacegroup name	P 32 2 1
Unit cell lengths	63.983, 63.983, 63.482
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.500
R-factor	0.199
Rwork	0.199
R-free	0.23000 *
RMSD bond length	0.004
RMSD bond angle	1.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.050 *	0.191
Number of reflections	24356 *
<I/σ(I)>	11.5
Completeness [%]	99.2	99.6
Redundancy	9	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	25 *	ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)	pH8.0
2	1	drop	EDTA	1 (mM)
3	1	reservoir	sodium acetate	100 (mM)	pH6.0
4	1	reservoir	ammonium sulfate	1.5 (M)
5	1	reservoir		2 (M)