1IZM
Structure of ygfB from Haemophilus influenzae (HI0817), a Conserved Hypothetical Protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97946, 0.97961, 0.96863 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.300, 42.300, 198.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| Rwork | 0.194 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.050 | 0.097 |
| Total number of observations | 298675 * | |
| Number of reflections | 24638 * | |
| <I/σ(I)> | 21.1 | |
| Completeness [%] | 98.0 * | 92 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | 1.4 M ammonimum sulfate, 0.05 M potassium phosphate, 3% iso-propanol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | pH7.0 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | reservoir | ammonium sulfate | 1.4 (M) | |
| 4 | 1 | reservoir | potassium phosphate | 0.05 (M) | pH4.8 |
| 5 | 1 | reservoir | isopropanol | 4 (%) |
