1IZL
Crystal Structure of Photosystem II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 129.377, 225.192, 308.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.700 |
| R-factor | 0.53045 |
| Rwork | 0.530 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.900 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.071 | 0.261 |
| Number of reflections | 94859 * | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 98.0 | 95 |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.5 | 20 * | Shen, J.R., (2000) Biochemistry, 39, 14739. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 4-5 (mg/ml) | |
| 2 | 1 | 1 | 20 (mM) | ||
| 3 | 1 | 1 | 8 (mM) | ||
| 4 | 1 | 2 | PEG1450 | 6-7 (%) | |
| 5 | 1 | 2 | 40 (mM) | ||
| 6 | 1 | 2 | 20 (mM) | ||
| 7 | 1 | 2 | MES | 20 (mM) | pH6.5 |
| 8 | 1 | 2 | 20 (mM) | ||
| 9 | 1 | 2 | n-dodecyl beta-D-maltoside | 0.02 (%) |
