1IZB
ROLE OF B13 GLU IN INSULIN ASSEMBLY: THE HEXAMER STRUCTURE OF RECOMBINANT MUTANT (B13 GLU-> GLN) INSULIN
Experimental procedure
| Spacegroup name | H 3 |
| Unit cell lengths | 82.850, 82.850, 34.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| R-factor | 0.18 |
| RMSD bond length | 0.020 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.050 * |
| Number of reflections | 5369 * |
| Completeness [%] | 91.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 0.02 (M) | ||
| 2 | 1 | 1 | zinc acetate | 2 (mg/ml) | |
| 3 | 1 | 1 | Na3 citrate | 50 (mM) | |
| 4 | 1 | 1 | acetone | 16 (%(v/v)) |
