1IZ6

Crystal Structure of Translation Initiation Factor 5A from Pyrococcus Horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-20
Detector	MARRESEARCH
Wavelength(s)	0.9
Spacegroup name	P 32
Unit cell lengths	93.280, 93.280, 39.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.000
Rwork	0.185
R-free	0.23600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkb
RMSD bond length	0.009
RMSD bond angle	26.760 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000 *	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.081	0.349
Total number of observations	148604 *
Number of reflections	25949
<I/σ(I)>	8.5	2.4
Completeness [%]	100.0	99.9
Redundancy	5.7	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	18 *	PEG 4000, isopropanol, Hepers, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES-NaOH	0.1 (M)	pH7.5
3	1	reservoir	isopropanol	10 (%)
4	1	reservoir	PEG4000	20 (%)
5	1	drop	protein	10 (mg/ml)