1IZ4

Pyrococcus furiosus PCNA mutant (Met73Leu/Asp143Ala): tetragonal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL24XU
Synchrotron site	SPring-8
Beamline	BL24XU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-11-14
Detector	RIGAKU RAXIS V
Wavelength(s)	0.836
Spacegroup name	P 43 21 2
Unit cell lengths	64.994, 64.994, 156.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.000
Rwork	0.270
R-free	0.29860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ge8
RMSD bond length	0.006
RMSD bond angle	1.247
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.529
Total number of observations	91731 *
Number of reflections	22310
<I/σ(I)>	10	1.6
Completeness [%]	95.9	97.4
Redundancy	4.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	20 *	ammonium sulfate, sodium citrate, glycerol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	100 (mM)	pH4.5
2	1	reservoir	ammonium sulfate	0.6 (M)
3	1	reservoir	D143A	40 (%(v/v))