1IZ3

Dimeric structure of FIH (Factor inhibiting HIF)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 6B
Synchrotron site	PAL/PLS
Beamline	6B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-07-09
Detector	MACSCIENCE
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	86.890, 86.890, 143.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.000 * - 2.800
R-factor	0.236
Rwork	0.230
R-free	0.27500 *
Structure solution method	MAD
RMSD bond length	0.008 *
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	50.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.072 *	0.195 *
Number of reflections	13563 *	98205 *
Completeness [%]	95.9 *	89.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5 *	25 *	0.4M Lithium sulfate, 20% PEG 4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	lithium sulfate	0.4 (M)
3	1	reservoir	PEG4000	20 (%)
4	1	reservoir	Tris	0.1 (M)	pH8.5