1IZ0
Crystal Structures of the Quinone Oxidoreductase from Thermus thermophilus HB8 and Its Complex with NADPH
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-09-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9791, 0.9793, 0.9000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 77.687, 77.687, 236.629 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 * - 2.300 |
Rwork | 0.224 |
R-free | 0.24900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 24.500 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.073 * | 0.148 * |
Total number of observations | 383738 * | |
Number of reflections | 20471 * | |
Completeness [%] | 97.6 | 76.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 293 | Shimomura, Y., (2002) Acta Crystallogr.,Sect.D, 58, 1365. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.4 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) |