1IYW
Preliminary Structure of Thermus thermophilus Ligand-Free Valyl-tRNA Synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | OXFORD PX210 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 186.562, 207.328, 59.235 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 4.000 |
| R-factor | 0.3196 |
| Rwork | 0.316 |
| R-free | 0.38280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.070 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.144 | 0.396 |
| Number of reflections | 65363 | |
| <I/σ(I)> | 7 | 2.4 |
| Completeness [%] | 93.8 | 89.4 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG4000, isopropanol, lithium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
