1IYO

Toho-1 beta-Lactamase In Complex With Cefotaxime

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	IMAGE PLATE
Detector	FUJI
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	72.581, 72.581, 98.179
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 1.800
Rwork	0.178
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.330 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.800
Rmerge	0.073
Total number of observations	96844 *
Number of reflections	26985
Completeness [%]	95.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	15 *	Ibuka, A., (1999) J. Mol. Biol., 285, 2079. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.8 (M)
2	1	reservoir	acetate	100 (mM)	pH4.5-4.7
3	1	drop	protein	15 (mg/ml)
4	1	drop	Tris-HCl	5 (mM)	pH7.0