1IYN

Crystal structure of chloroplastic ascorbate peroxidase from tobacco plants and structural insights for its instability

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-06-07
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	37.188, 76.790, 98.776
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 1.600
R-factor	0.191
Rwork	0.191
R-free	0.22300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1apx
RMSD bond length	0.006
RMSD bond angle	20.900 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.066 *	0.243 *
Total number of observations	1018666 *
Number of reflections	37796 *
<I/σ(I)>	8.1	3.1
Completeness [%]	98.9 *	98.9 *
Redundancy	6.5 *	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	277	Wada, K., (2002) Acta Crystallogr., D58, 559. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	50 (mM)	pH7.0
2	1	reservoir	AsA	1 (mM)
3	1	drop	protein	8 (mg/ml)