1IYL
Crystal Structure of Candida albicans N-myristoyltransferase with Non-peptidic Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 288 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-11-28 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.277, 96.885, 269.277 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| R-factor | 0.288 |
| Rwork | 0.284 |
| R-free | 0.36600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iyk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.072 | 0.364 |
| Number of reflections | 40711 | |
| <I/σ(I)> | 16.4 | 3.1 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | PEG3350, lithium sulfate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 60 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 15-18 (%(w/v)) | |
| 3 | 1 | reservoir | lithium sulfate | 0.2 (M) | |
| 4 | 1 | reservoir | HEPES | 50 (mM) | pH7.5 |
