1IYK
Crystal structure of candida albicans N-myristoyltransferase with myristoyl-COA and peptidic inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 288 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-11-02 |
| Detector | WEISSENBERG |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.696, 95.334, 176.549 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.300 |
| R-factor | 0.206 |
| Rwork | 0.192 |
| R-free | 0.22300 * |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.268 |
| Number of reflections | 41498 | |
| <I/σ(I)> | 23.8 | 3.2 |
| Completeness [%] | 92.2 * | 71.4 |
| Redundancy | 4.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | PEG3350, ammonium acetate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 60 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 10-12 (%(w/v)) | |
| 3 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 4 | 1 | reservoir | HEPES | 50 (mM) | pH7.5 |
