1IYB

Crystal Structure of the Nicotiana glutinosa Ribonuclease NW

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Detector	OXFORD PX210
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	44.800, 94.300, 98.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.400 * - 1.500
Rwork	0.212
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dix
RMSD bond length	0.005
RMSD bond angle	23.400 *
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.400 *	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.052	0.201
Number of reflections	62367
<I/σ(I)>	28.6	2.7
Completeness [%]	93.2	53.8
Redundancy	5.6	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7 *	18 *	ammonium dihydrogen, Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium phosphate	10 (mM)	pH7.0
3	1	reservoir	5'-GMP	10 (mM)