1IY0

Crystal structure of the FtsH ATPase domain with AMP-PNP from Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-06-04
Detector	MACSCIENCE
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	73.872, 73.872, 89.253
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 2.950
Rwork	0.225
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009 *
RMSD bond angle	0.009
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	2.950	2.950
Rmerge	0.081 *	0.276 *
Number of reflections	5851
Completeness [%]	98.8	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	20 *	Ammonium sulfate, Tris-HCl, AMP-PNP, magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)	pH8.0
2	1	reservoir	ammonium sulfate	1.6 (M)
3	1	reservoir	glycerol	20 (%(v/v))
4	1	drop	AMP-PNP	100 (mM)
5	1	drop		40 (mM)