1IXZ

Crystal structure of the FtsH ATPase domain from Thermus thermophilus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-31
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0059, 1.0090, 1.0139
Spacegroup name	P 65
Unit cell lengths	73.535, 73.535, 88.468
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 2.200
Rwork	0.196
R-free	0.23800
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.064 *	0.285 *
Number of reflections	13870
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	20 *	Ammonium sulfate, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	0.1 (M)	pH8.0
2	1	reservoir	ammonium sulfate	1.6 (M)
3	1	reservoir	glycerol	20 (%(v/v))
4	1	drop	AMP-PNP	100 (mM)
5	1	drop		40 (mM)