1IXK
Crystal Structure Analysis of Methyltransferase Homolog Protein from Pyrococcus Horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9000, 0.9805, 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.554, 41.513, 71.065 |
| Unit cell angles | 90.00, 114.18, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| Rwork | 0.199 |
| R-free | 0.23500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.523 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.770 * | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.097 | 0.329 * |
| Total number of observations | 152004 * | |
| Number of reflections | 26745 | |
| <I/σ(I)> | 6.8 | 2.6 |
| Completeness [%] | 99.0 | 98.6 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5 | 20 * | PEG6000, Citric Acid, Glyserol, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | citric acid | 0.1 (M) | pH5. |
| 3 | 1 | reservoir | PEG6000 | 15 (%) | |
| 4 | 1 | reservoir | 1-5 pentandiol | 1 (%) | |
| 5 | 1 | reservoir | glycerol | 5 (%) |
