1IXH
PHOSPHATE-BINDING PROTEIN (PBP) COMPLEXED WITH PHOSPHATE
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-01 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.506, 63.379, 122.373 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.400 - 0.980 |
R-factor | 0.114 |
R-free | 0.14100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ABH |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXL-96 |
Refinement software | SHELXL-96 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.400 | 1.000 |
High resolution limit [Å] | 0.980 | 0.980 |
Rmerge | 0.053 | 0.246 |
Number of reflections | 147105 | |
<I/σ(I)> | 35.3 | 3.6 |
Completeness [%] | 79.8 | 29.8 |
Redundancy | 9.4 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 | Wang, Z., (1994) J.Biol.Chem., 269, 25091. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.25 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 1.5 (mM) | |
3 | 1 | drop | PEG6000 | 13.5 (%(w/v)) | |
4 | 1 | drop | 37.5 (mM) | ||
5 | 1 | drop | potassium acetate | 15 (mM) | |
6 | 1 | reservoir | PEG6000 | 18 (%(w/v)) | |
7 | 1 | reservoir | 50 (mM) | ||
8 | 1 | reservoir | potassium phosphate | 2 (mM) | |
9 | 1 | reservoir | potassium acetate | 20 (mM) |