1IX0

I59A-3SS human lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-03-27
Detector	RIGAKU RAXIS IV
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	56.830, 60.880, 32.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
Rwork	0.179
R-free	0.25600 *
Structure solution method	isomorphous
RMSD bond length	0.009 *
RMSD bond angle	1.490 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800
Rmerge	0.041 *	0.056 *
Total number of observations	50796 *
Number of reflections	10772
Completeness [%]	99.0	98.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	10 *	Takano, K., (1995) J.Mol.Biol., 254, 62. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir		2.5 (M)
3	1	reservoir	acetate	20 (mM)