1IWM
Crystal Structure of the Outer Membrane Lipoprotein Receptor, LolB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.998 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.190, 112.440, 47.790 |
| Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| Rwork | 0.215 |
| R-free | 0.24900 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 * | 0.279 * |
| Number of reflections | 28181 | |
| Completeness [%] | 97.9 | 95 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * | PEGMME2000, sodium acetate, ammonium sulfate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
| 3 | 1 | reservoir | PEG2000MME | 30 (%(w/v)) | |
| 4 | 1 | reservoir | sodium acetate | 0.1 (mM) | pH4.6 |
| 5 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
