1IWG
Crystal structure of Bacterial Multidrug Efflux transporter AcrB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-10 |
| Detector | MAC Science DIP-2040 |
| Wavelength(s) | 0.900 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 144.536, 144.536, 519.178 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 3.500 |
| Rwork | 0.290 |
| R-free | 0.35500 |
| Structure solution method | MIR |
| RMSD bond length | 0.003 * |
| RMSD bond angle | 0.867 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.090 | 0.365 |
| Total number of observations | 198425 * | |
| Number of reflections | 26406 * | 2584 * |
| <I/σ(I)> | 11.5 | 2.6 |
| Completeness [%] | 98.8 | 98.6 |
| Redundancy | 7.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 * | 25 * | PEG 2000, Sodium phosphate, sodium citrate, glycerol, dodecyl maltoside, pH 6.1, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 28 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 20 (mM) | pH6.2 |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | dodecylmaltoside | 0.2 (%) | |
| 5 | 1 | reservoir | PEG2000 | 15-16 (%) | |
| 6 | 1 | reservoir | sodium phosphate | 80 (mM) | pH6.2 |
| 7 | 1 | reservoir | sodium citrate-HCl | 20 (mM) | pH5.6 |
