1IW2

X-ray structure of Human Complement Protein C8gamma at pH=7.O

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	94
Detector technology	IMAGE PLATE
Collection date	1999-08-03
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	42.140, 58.904, 71.214
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 1.900
R-factor	0.239 *
Rwork	0.233
R-free	0.25500 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	26.500 *
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.970
High resolution limit [Å]	1.790 *	1.900
Rmerge	0.044 *	0.138
Number of reflections	13149 *
Completeness [%]	75.8 *	13.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2 *	4 *	PEG 4000, sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	imidazole	25 (mM)
2	1	drop		150 (mM)	pH7.2
3	1	drop	protein	0.3-0.5 (mg/ml)
4	1	reservoir	PEG4000	28-36 (%)
5	1	reservoir	sodium citrate	0.1 (M)	pH4.0