1IW0
Crystal structure of a heme oxygenase (HmuO) from Corynebacterium diphtheriae complexed with heme in the ferric state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2001-12-06 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.559, 62.838, 107.661 |
| Unit cell angles | 90.00, 100.86, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.400 |
| R-factor | 0.168 |
| Rwork | 0.165 |
| R-free | 0.19200 * |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.740 * |
| Data reduction software | JUPITER210 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.340 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.034 | 0.297 |
| Total number of observations | 542325 * | |
| Number of reflections | 146822 | |
| <I/σ(I)> | 18.08 | 0.87 |
| Completeness [%] | 85.0 | 58.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 303 | ammonium sulfate, NaI, MES, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 303K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | MES | 20 (mM) | pH7.0 |
| 3 | 1 | reservoir | MES | 50 (mM) | pH5.8 |
| 4 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 5 | 1 | reservoir | sodium iodode | 230 (mM) |
