1IVP
THE CRYSTALLOGRAPHIC STRUCTURE OF THE PROTEASE FROM HUMAN IMMUNODEFICIENCY VIRUS TYPE 2 WITH TWO SYNTHETIC PEPTIDIC TRANSITION STATE ANALOG INHIBITORS
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.190, 44.950, 135.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.500 |
| R-factor | 0.197 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.043 * |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 * |
| Total number of observations | 20722 * |
| Number of reflections | 5285 * |
| Completeness [%] | 86.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 6 (mg/ml) | |
| 2 | 1 | drop | MOPS | 0.1 (M) | |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | ethylene glycol | 5 (%) | |
| 5 | 1 | reservoir | PEG4000 | 25-30 (%) | |
| 6 | 1 | reservoir | HEPES | 0.1 (M) | |
| 7 | 1 | reservoir | 1-butanol | 3-4 (%) |
