1IVI
Crystal Structure of pig dihydrolipoamide dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Wavelength(s) | 1.04 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 359.300, 359.300, 140.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 8.000 |
| Rwork | 0.378 |
| R-free | 0.37900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 8.000 |
| Rmerge | 0.066 |
| Total number of observations | 13615 * |
| Number of reflections | 5838 |
| Completeness [%] | 98.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Ammonium sulphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | potassium phosphate | 50 (mM) | pH7.0 |
| 2 | 1 | drop | protein | 6.6 (mg/ml) | |
| 3 | 1 | drop | FAD | 0.25 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 0.75 (M) | |
| 5 | 1 | reservoir | potassium phosphate | 50 (mM) | pH7.0 |
| 6 | 1 | reservoir | ammonium sulfate | 1.5 (M) |
