1IV9
Crystal Structure of Single Chain Monellin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200H |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-20 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.120, 76.400, 45.910 |
| Unit cell angles | 90.00, 99.50, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.18326 |
| Rwork | 0.181 |
| R-free | 0.21706 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mol |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.199 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.033 | 0.177 |
| Number of reflections | 15522 | |
| Completeness [%] | 95.7 | 90.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.3 | 277 | PEG8000, HEPES, pH 7.3, micro batch, temperature 277K |
