1IV8
Crystal Structure of Maltooligosyl trehalose synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-16 |
| Detector | FUJI |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.504, 68.497, 93.769 |
| Unit cell angles | 90.00, 101.46, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| Rwork | 0.197 |
| R-free | 0.25600 |
| Structure solution method | MIR |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.084 | |
| Number of reflections | 60100 | |
| Completeness [%] | 92.3 | 89.3 |
| Redundancy | 1.164 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 2000, MAGNESIUM CHLORIDE, TRIS, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
