1IUZ

PLASTOCYANIN

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Detector technology	DIFFRACTOMETER
Collection date	1996-06-22
Detector	WEISSENBERG
Spacegroup name	P 43 3 2
Unit cell lengths	88.300, 88.300, 88.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.600
R-factor	0.176
Rwork	0.176
R-free	0.21100
RMSD bond length	0.012
RMSD bond angle	29.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.600 *	1.600 *
Rmerge	0.082
Total number of observations	115971 *
Number of reflections	14759
Completeness [%]	0.9	0.702 *
Redundancy	7.86

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	glycine	50 (mM)
3	1	drop	ammonium sulfate	1.1 (M)
4	1	reservoir	glycine	100 (mM)
5	1	reservoir	ammonium sulfate	2.2 (M)