1IUQ
The 1.55 A Crystal Structure of Glycerol-3-Phosphate Acyltransferase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.138, 63.604, 115.862 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.550 |
| R-factor | 0.20216 |
| Rwork | 0.201 |
| R-free | 0.21700 * |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.862 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.125 | 1.610 |
| High resolution limit [Å] | 1.550 * | 1.550 * |
| Rmerge | 0.035 * | 0.292 * |
| Number of reflections | 61882 * | |
| <I/σ(I)> | 9.2 | 1.5 |
| Completeness [%] | 97.7 | 86.6 |
| Redundancy | 7.1 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 277 | 1.6-1.8M AMMONIUM SULFATE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium phosphate | 20 (mM) | pH8.0 |
| 2 | 1 | drop | 0.5 (M) | ||
| 3 | 1 | drop | protein | 14 (mg/ml) | |
| 4 | 1 | drop | sodium phosphate | 20 (mM) | pH8.0 |
| 5 | 1 | reservoir | PEG4000 | 4-25 (%) | |
| 6 | 1 | reservoir | ammonium acetate | 100 (mM) | |
| 7 | 1 | reservoir | 2-propanol | 10 (%(v/v)) | |
| 8 | 1 | reservoir | citrate | 100 (mM) | pH5.6 |
