1IUD
MALTODEXTRIN-BINDING PROTEIN INSERTION/DELETION MUTANT WITH AN INSERTED B-CELL EPITOPE FROM THE PRES2 REGION OF HEPATITIS B VIRUS
Experimental procedure
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 288 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.100, 97.400, 137.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.700 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | WILD-TYPE MALTOSE-BINDING PROTEIN |
RMSD bond length | 0.012 |
RMSD bond angle | 24.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.054 | 0.367 |
Number of reflections | 11201 | 1108 * |
<I/σ(I)> | 14.3 | 3.1 |
Completeness [%] | 99.0 | 97 |
Redundancy | 3.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 * | 17 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG6000 | 15 (%) | can be replaced by PEG8000 |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | 3 (mM) | ||
5 | 1 | drop | maltose | 1 (mM) | |
6 | 1 | drop | protein | 3 (mg/ml) | |
7 | 1 | reservoir | PEG | 22 (%) |