1IUC
Fucose-specific lectin from Aleuria aurantia with three ligands
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-16 |
| Detector | FUJI |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 84.032, 84.032, 250.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 * - 2.240 |
| Rwork | 0.164 |
| R-free | 0.19600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 * | 2.290 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.061 | 0.301 |
| Number of reflections | 46319 | |
| <I/σ(I)> | 31.3 | |
| Completeness [%] | 91.2 | 81.2 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 297 * | Fujihashi, M., (2003) Acta Crystallogr.,Sect.D, 59, 378. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | sodium phosphate | 8 (mM) | |
| 3 | 1 | reservoir | potassium phosphate | 1.5 (mM) | |
| 4 | 1 | reservoir | 137 (mM) | ||
| 5 | 1 | reservoir | 2.7 (mM) | pH7.2 | |
| 6 | 1 | reservoir | beta-mercaptoethanol | 20 (mM) |
