1IU8
The X-ray Crystal Structure of Pyrrolidone-Carboxylate Peptidase from Hyperthermophilic Archaeon Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.899, 66.479, 76.396 |
| Unit cell angles | 90.00, 128.68, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.189 * |
| Rwork | 0.189 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iof |
| RMSD bond length | 0.004 |
| RMSD bond angle | 22.920 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.600 | 1.686 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.061 * | 0.269 * |
| Total number of observations | 207328 * | |
| Number of reflections | 54101 | |
| <I/σ(I)> | 8 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 4 * | MPD, Magnesium Chlolide, Imidazole, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | imidazole | 100 (mM) | pH7.4-9.0 |
| 2 | 1 | reservoir | MPD | 35-38 (%) | |
| 3 | 1 | reservoir | 0.1-0.2 (M) | ||
| 4 | 1 | drop | protein | 10 (mg/ml) |
