1IU5
X-ray Crystal Structure of the rubredoxin mutant from Pyrococcus Furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-29 |
| Detector | MAC Science DIP-2000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.480, 35.700, 43.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| Rwork | 0.187 |
| R-free | 0.20400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1brf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.077 | 0.190 |
| Total number of observations | 38773 * | |
| Number of reflections | 8876 | |
| <I/σ(I)> | 25.2 | |
| Completeness [%] | 98.6 | 97.7 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | used macroseeding, Bau, R., (1998) J.BIOL.INORG.CHEM., 3, 484. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 0.3 (M) | ||
| 4 | 1 | reservoir | sodium potassium phosphate | 3.6 (M) |
