1IU1

Crystal structure of human gamma1-adaptin ear domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-09-18
Detector	OXFORD PX210
Wavelength(s)	0.900
Spacegroup name	P 43 21 2
Unit cell lengths	62.132, 62.132, 147.869
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.22756
Rwork	0.226
R-free	0.24800 *
RMSD bond length	0.009
RMSD bond angle	1.330 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.000	1.890
High resolution limit [Å]	1.800	1.800
Rmerge	0.064 *	0.219 *
Total number of observations	372969 *
Number of reflections	27518
Completeness [%]	99.2	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *	277	PEG4000, magnesium chloride, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	unknown *	7.5 *	277	PEG4000, magnesium chloride, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	unknown *	7.5 *	277	PEG4000, magnesium chloride, HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	PEG4000	20 (%(w/v))
3	1	drop		200 (mM)
4	1	drop	HEPES-Na	100 (mM)	pH7.5