1ITZ

Maize Transketolase in complex with TPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2001-04-20
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	136.438, 136.438, 203.731
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.520 - 2.300
R-factor	0.167
Rwork	0.166
R-free	0.20000 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008 *
RMSD bond angle	1.460 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.520	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.120 *	0.360 *
Number of reflections	91552 *
Completeness [%]	93.8 *	93.8
Redundancy	3.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	20 *	PEG 3350, ammonium acetate, spermine, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12-15 (mg/ml)
2	1	drop	Tris hydrochloride	10 (mM)	pH7.5
3	1	drop		2 (mM)
4	1	drop	TPP	50000 (nM)
5	1	reservoir	PEG3350	13 (%(w/v))
6	1	reservoir	ammonium acetate	130 (mM)
7	1	reservoir	spermine	100 (mM)