1ITV
Dimeric form of the haemopexin domain of MMP9
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 43 |
| Unit cell lengths | 127.720, 127.720, 31.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.232 |
| Rwork | 0.229 |
| R-free | 0.27500 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.082 | |
| Total number of observations | 124981 * | |
| Number of reflections | 36949 | |
| Completeness [%] | 97.8 | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | Tris, PEGMME, ammonium sulphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | Tris, PEGMME, ammonium sulphate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.5 |
| 3 | 1 | reservoir | PEG5000 MME | 30 (%(w/v)) | |
| 4 | 1 | reservoir | ammonium sulfate | 200 (mM) |
