1IT7

Crystal structure of archaeosine tRNA-guanine transglycosylase complexed with guanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45PX
Synchrotron site	SPring-8
Beamline	BL45PX
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-02-26
Detector	RIGAKU RAXIS V
Wavelength(s)	1.02
Spacegroup name	P 43 21 2
Unit cell lengths	100.201, 100.201, 364.658
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.2291 *
Rwork	0.229
R-free	0.27120 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iq8
RMSD bond length	0.007
RMSD bond angle	1.330 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.107	0.382
Total number of observations	567949 *
Number of reflections	82107 *	3973 *
<I/σ(I)>	19.6	3.78
Completeness [%]	98.4	96.7
Redundancy	6.9	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Ishitani, R., (2001) Acta Cryst., D57, 1659. *