1ISY
Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with glucose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-05-15 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.250, 94.470, 138.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.100 |
R-factor | 0.193 * |
Rwork | 0.191 |
R-free | 0.23300 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xyf |
RMSD bond length | 0.005 |
RMSD bond angle | 1.290 * |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | CrystalClear ((MSC/RIGAKU)) |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.100 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.129 | 0.320 |
Total number of observations | 341605 * | |
Number of reflections | 92029 * | |
<I/σ(I)> | 10 | 4.5 |
Completeness [%] | 89.9 | 68.8 |
Redundancy | 3.85 | 3.77 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 298 | ammonium sulfate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 25 (%(v/v)) | or 27.5%(v/v) |
3 | 1 | reservoir | citric acid | 0.1 (M) | |
4 | 1 | reservoir | sodium phosphate | 0.2 (M) | pH5.7 |